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Publication of the team of prof. Małgorzata Barańska (Jagiellonian University, Scientific Council of the IOC PAS) and the Team of prof. Jacek Młynarski at Biosensors & Bioelectronics (IF 12.5)
EdU (5-ethynyl-2′-deoxyuridine) is an alkyne-functionalized proliferation probe that integrates into newly synthesized DNA for fluorescence imaging of cell proliferation using a click reaction with a fluorescent azide. The authors of the study used EdU as a Raman probe without requiring an azide reaction to track endothelial cell proliferation. The published results show that EdU imaging based on Raman spectroscopy is a new alternative to the fluorescence-based assay for the assessment of endothelial proliferation and regeneration.