Laboratory of Nuclear Magnetic Resonance
dr hab. Wojciech Schilf, associate professor
phone +48 (22) 343 33 18
History of the Nuclear Magnetic Resonance lab begun at the beginning of 1962, when Institute got a first spectrometer, Varian 60 MHz. It is worth to notice that it was the first spectrometer of nuclear magnetic resonance in Poland. From that moment our lab started to work for Institute and whole scientific environment. The purchase of high-field spectrometer Bruker AM 500 at the end of 1987 was the next turning point in lab's work. This spectrometer allowed us to reach the word standard of measurements, and initiated a new period in our lab. Now, we are equipped with five NMR spectrometers, from the simplest apparatus Varian Gemini 200 to the newest Varian VNMRS 600. These instruments allow us to fulfil the most sophisticated analytical requirements of our clients.
Our offer includes a full range of one- and two-dimensional nuclear magnetic resonance experiments in the liquid phase and CPMAS measurements in the solid phase for most of nuclei with non-zero spin.
Typically, we perform the measurements at room temperature, but the experiments at various temperatures, depending on a probe and solvent, are also available.
All probes of spectrometers Varian 500 and 600 MHz allow the measurements in the range of temperatures from -120 to +120 oC.
The lab possesses a whole range of deuterated solvents. We also aid our clients in the selection of suitable experiments and spectra analysis.
Now the lab have the following spectrometers:
Varian Gemini 200 MHz – Varian Gemini 200 MHz – an instrument designed for routine ¹H and ¹³C measurements at room temperature; this spectrometer works as self-service machine, available for each trained person. Practically, the spectrometer is available for most PhD students and employees from out Institute and other centres.
Varian Mercury 400 MHz - this apparatus works also as a self-service machine. The spectrometer is equipped with a four-nuclei probe, allowing measuring of ¹H, 19F, ¹³C and ³¹P spectra, with no need of probe tuning. The configuration of this apparatus allows the performing of most standard one- and two-dimensional NMR experiments.
Bruker DRX 500 MHz – An all-purpose NMR spectrometer, designed for measurements in liquid and solid phases. The probes of the spectrometer allow the measurements in a large range of temperatures, from -120 to +150 oC. The spectrometer is equipped with the following probes:
- DUAL – 5 mm - a probe dedicated for ¹H and ¹³C measurements by direct method; this probe is especially useful for ¹³C NMR, which demands prolonged spectra acquisition.
- TBI – 5 mm – a three-channel, inverse z-gradient probe, equipped with the ¹H channel, ¹³C channel and BB channel working in the range from P to Ag. The probe is designed for all kinds of correlation experiments requiring gradient techniques and ¹H detection. Due to high sensitivity of the ¹H channel, the probe is recommended for all ¹H NMR experiments.
- Broadband – 5 mm - a two-channel, inverse gradient probe, containing the ¹H detection channel and broadband channel, covering the range from P to Ag. The probe is useful for heteroatom correlation experiments with ¹H detection.
- N-15 – 10 mm – a probe dedicated to 15N measurements by the direct method with 1H decoupling.
- Broadband – 10 mm -a two-channel probe equipped with the ¹H channel and broadband channel covering the range from P to Ag. The probe designed for heteronuclear measurements by the direct method with ¹H decoupling.
- CP-MAS – 4 mm - a two-channel broadband probe, designed for measurements in the solid phase, by cross-polarization technique. The broadband channel covers the range from 31P to 15N.
Varian VNMRS 500 MHz – A spectrometer of the newest generation, able to perform the full range of NMR experiments in the liquid phase. The spectrometer is equipped with two probes:
- AutoX ID – 5 mm - a two-channel inverse gradient probe, with the 1H detection channel and broadband channel covering the range from 31P to 15N. Due to the high sensitivity of the 1H channel, the probe is dedicated for 1H experiments and heteronuclear correlation experiments by inverse techniques.
- AutoX DB – 5mm - a two-channel gradient probe with broadband channel covering the range from 31P to 15N. The probe dedicated for the direct measurement of heteronuclei.
Varian VNMRS 600 MHz – An all-purpose spectrometer of the newest generation, designed for the measurements in the liquid and solid phases. The spectrometer is equipped with the following probes:
- AutoX ID – 5mm - - a two-channel inverse probe, equipped with the 1H detection channel and broadband channel covering the range from 31P to 15N. Due to high sensitivity of the 1H channel, the probe is especially suitable for 1H experiments and heteronuclear correlation experiments by inverse techniques.
- AutoX DB – 5mm - a two-channel probe, covering the range from 31P to 15N, especially designed for the direct measurements of heteronuclei.
- Tunable Triple Probe – 5mm – a three-channel inverse probe equipped with the gradient coil, 1H detection channel, selective 31P channel and broadband channel covering the range from 15N to 103Rh. The probe designed for the measurement of nuclei with low resonance frequencies.
- CPMAS – 3,2 mm – a two-channel probe designed for measurements in the solid phase. The broadband channel covers the range from 31P to 15N.